![]() ![]() This variation is used in cancer detection. However, limiting a primer also decreases the annealing temperature affecting reaction efficiency (avoided using LATE-PCR).Ĭo-Amplification at Lower Denaturation Temperature (COLD) -PCR: Variant alleles are amplified from a mixture wild-type and minority mutation-containing DNA. Upon depletion of the limited primer, DNA synthesis of the other strand proceeds arithmetically rather than exponentially (as in conventional PCR). This is done by limiting or leaving out one of the primers. Primers with an overlap are used to create products that can then be used as a template to generate the long DNA molecules.Īsymmetric PCR: One strand of DNA is preferentially amplified. This is used in SNP genotyping.Īssembly ( Polymerase Cycling Assembly or PCA) PCR : Long DNA molecules are created from long oligonucleotides with short overlapping segments. Since mismatched primers will not initiate replication whereas matched primers will, amplification is indicative of the mutation being present. This article lists some variants of PCR alphabetically in the hope of creating an awareness of the variations that have been created for very specific purposes but may have other applications, as well as to assist in increasing awareness of the broad range of applications for this technique in general.Īllele-specific PCR: Rather than designing primers for an invariant part of the genome in order to amplify a more polymorphic area between them, at least one of the primers used in this variation of PCR is complementary to a polymorphic area, with mutations located at its 3’ end. While some are optimizations to suit specific requirements and are very similar to basic PCR, others completely turn the technique on its head to formulate novel creative applications in various fields. The wide range of applications of PCR has led to an ever-growing list of variants of the technique.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |